Comparison of Antibacterial Activities of Garlic and Goldenseal Infused ZnO Nanoparticles
Additional Funding Sources
The project described was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grant No. P20GM103408.
Abstract
In response to the increasing antibiotic-resistance of bacteria, many medical researchers have turned to metal nanoparticles as a different method of attack against bacterial infections. Published data suggests that infusing antibacterial plant extracts during the synthesis of ZnO nanoparticles results in more inhibition of bacteria compared to the ZnO NP alone. Three experiments were conducted using ZnO nanoparticles infused with garlic and goldenseal plant extracts against E. coli and S. aureus bacteria. Experiments to determine the growth curve and minimum inhibitory concentrations (MIC) were performed in 96-well plates with serially diluted concentrations of NP's and incubated for 10 or 20 hours. Transfer of broth to a solid medium was used to identify the minimum bactericidal concentration (MBC). Growth over time was measured using spectrophotometry over 10 hour periods. Limited inhibition against E. coli and S. aureus was observed through spectrophotometry from the microdilution assay, although visible growth was evident at each concentration. Complete growth on the solid medium signified no bactericidal effects from the NP's. All nanoparticles showed comparable inhibition over a 10 hour period. The research displayed a lack of efficacy for the antibacterial properties of ZnO NP's, infused or plain, against other antibacterial agents.
Comparison of Antibacterial Activities of Garlic and Goldenseal Infused ZnO Nanoparticles
In response to the increasing antibiotic-resistance of bacteria, many medical researchers have turned to metal nanoparticles as a different method of attack against bacterial infections. Published data suggests that infusing antibacterial plant extracts during the synthesis of ZnO nanoparticles results in more inhibition of bacteria compared to the ZnO NP alone. Three experiments were conducted using ZnO nanoparticles infused with garlic and goldenseal plant extracts against E. coli and S. aureus bacteria. Experiments to determine the growth curve and minimum inhibitory concentrations (MIC) were performed in 96-well plates with serially diluted concentrations of NP's and incubated for 10 or 20 hours. Transfer of broth to a solid medium was used to identify the minimum bactericidal concentration (MBC). Growth over time was measured using spectrophotometry over 10 hour periods. Limited inhibition against E. coli and S. aureus was observed through spectrophotometry from the microdilution assay, although visible growth was evident at each concentration. Complete growth on the solid medium signified no bactericidal effects from the NP's. All nanoparticles showed comparable inhibition over a 10 hour period. The research displayed a lack of efficacy for the antibacterial properties of ZnO NP's, infused or plain, against other antibacterial agents.