Effects of Sodium Nitroprusside on Mouse Erythrocyte Catalase Activity and Malondialdehyde Status

Authors

Mamane Sani, Faculté des Sciences et Techniques de Maradi
Hichem Sebai, UR Ethnobotanie et Stress Oxydant
Roberto Refinetti, Boise State UniversityFollow
Mohan Mondal, National Dairy Research Institute
Néziha Ghanem-Boughanmi, UR Ethnobotanie et Stress Oxydant
Naceur A. Boughattas, Faculté de Médecine
Mossadok Ben-Attia, Faculté des Sciences de Bizerte

Document Type

Article

Publication Date

7-2016

Abstract

There is controversy about the anti- or pro-oxidative effects of the nitric oxide (NO)-donor sodium nitroprusside (SNP). Hence, the activity of the antioxidant enzyme catalase (CAT) and the status of malondialdehyde (MDA) were investigated after a 2.5 mg/kg dose of SNP had been i.p. administered to different and comparable groups of mice (n =  48). The drug was administered at two different circadian times (1 and 13 h after light onset [HALO]). There were, irrespectively of sampling time, no significant differences in the means of CAT activity and MDA status between control and SNP-treated groups, no matter the treatment time. However, CAT activity was significantly (Student’s t-test, p < 0.001) increased 1 h following SNP administration at 1 HALO, whereas the significant (p < 0.001) increase in the enzyme activity was found only 3 h after injection at 13 HALO. The drug dosing either at 1 or 13 HALO resulted in no significant differences of MDA status between control and treated groups regardless to the sampling time. Two-way analysis of variance (ANOVA) detected a significant (F_0.05(7,88)= 5.3; p < 0.0006) interaction between sampling time and treatment in mice injected at 1 HALO, suggesting the influence of treatment on sampling-time-related changes in CAT activity. However, ANOVA validated no interaction between the two factors in mice treated at 13 HALO, illustrating that the sampling-time differences in enzyme activity were greater. Furthermore, two-way ANOVA revealed no interaction in the variation of MDA status in animals treated either at 1 or 13 HALO. This study indicates that SNP significantly affected the anti-oxidant system.

Publication Information

Sani, Mamane; Sebai, Hichem; Refinetti, Roberto; Mondal, Mohan; Ghanem-Boughanmi, Néziha; Boughattas, Naceur A.; and Ben-Attia, Mossadok. (2016). "Effects of Sodium Nitroprusside on Mouse Erythrocyte Catalase Activity and Malondialdehyde Status". Drug and Chemical Toxicology, 39(3), 350-356. http://dx.doi.org/10.3109/01480545.2015.1122032