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Document Type

Abstract

Publication Date

12-1-2024

Abstract

Goal: With the improvement of living standards, people's diets tend to be more and more high-fat, and long-term high-fat diets may lead to obesity and other chronic health problems. Nearly 80% of obese patients are accompanied by varying degrees of hepatic steatosis, so there is an urgent need to seek a safe and effective treatment of obesity and lipid deposition.

Methods: In this study, 3-week-old Sprague-Dawley rats were divided into normal control group (NC), high-fat group: control group (HC), light fasting group (HI), exercise group (HE), light fasting plus exercise group (HIE), and light fasting, and aerobic exercise were used as intervention methods for 10 weeks. Catabolic protein effects were investigated to explore the effects of both on hepatic lipid deposition, and the experimental data were statistically analyzed using SPSS 25.0, with one-way or two-way ANOVA tests to be used, and graphs were made using GraphPad Prism 8.0.

Findings: (1) Weight was significantly higher in the HC group compared to the NC group (p < 0.01). Compared with the HC group, the intervention group had lower body weight (P < 0.05), with the HIE group having a greater reduction in body weight. (2) The results of HE and oil red staining after 10 weeks of intervention showed that the liver structure and histological morphology of high-fat rats underwent significant improvement (P < 0.05), and the effect was more significant in the HIE group. (3) Compared with the NC group, serum TG, TC, LDL, AST, ALT were elevated (P < 0.05) and HDL was reduced (P < 0.05) in the HC group; compared with the HC group, TG, TC, LDL, AST, ALT were reduced, and HDL was elevated (P < 0.05) in the intervention group. (4) Compared with the NC group, the expression of p-AMPK/AMPK, p-ACC/ACC, and CPT1 was significantly reduced in the liver tissues of mice in the HC group (P < 0.01). The expression of p-AMPK/AMPK, p-ACC/ACC, and CPT1 was elevated in the liver tissues of the intervention group compared with the HC group (P < 0.05).

Discussion: The present study confirmed that the effect of light fasting plus exercise on the improvement of lipid deposition was better than that of one alone, which provided a new idea and direction for the improvement of obesity and lipid deposition; however, this study did not go into depth on the mechanism at the molecular level, which needs to be continued to be explored.

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