Microbiota Compositional Changes in Mice Correlated with Tamiflu, Influenza and MRSA Co-Infection
Additional Funding Sources
This project is supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award No. R25GM123927. The project described was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grant No. P20GM103408.
Presentation Date
7-2020
Abstract
Influenza virus infection increases susceptibility to bacterial infection and mortality in humans. Oseltamivir (Tamiflu) is a neuraminidase inhibitor and reported to have the possible benefit in reducing mortality caused by coinfection. When a host is infected with Influenza A virus (IAV), there are dynamic changes in the gut microbiome. We hypothesize that Oseltamivir induces gut microbiome composition changes in mouse model coinfected with IAV and Methicillin Resistant Staphylococcus Aureus (MRSA). The fecal samples were analyzed with the quantitative PCR (qPCR) assay. Bacterial DNA was isolated through lysis and purification of mouse fecal samples. Primers were selected for the 8 common bacteria phylum found in mouse feces. The qPCR results were analyzed using PfaffI method to characterize the bacterial composition. Our data indicated a noticeable difference in the microbiome composition in the Tamiflu treatment group compared to the control group after IAV and MRSA coinfection. The analysis of the bacterial DNA isolated from mouse feces allowed us to validate our protocol and testing strategy for future mouse experiments.
Microbiota Compositional Changes in Mice Correlated with Tamiflu, Influenza and MRSA Co-Infection
Influenza virus infection increases susceptibility to bacterial infection and mortality in humans. Oseltamivir (Tamiflu) is a neuraminidase inhibitor and reported to have the possible benefit in reducing mortality caused by coinfection. When a host is infected with Influenza A virus (IAV), there are dynamic changes in the gut microbiome. We hypothesize that Oseltamivir induces gut microbiome composition changes in mouse model coinfected with IAV and Methicillin Resistant Staphylococcus Aureus (MRSA). The fecal samples were analyzed with the quantitative PCR (qPCR) assay. Bacterial DNA was isolated through lysis and purification of mouse fecal samples. Primers were selected for the 8 common bacteria phylum found in mouse feces. The qPCR results were analyzed using PfaffI method to characterize the bacterial composition. Our data indicated a noticeable difference in the microbiome composition in the Tamiflu treatment group compared to the control group after IAV and MRSA coinfection. The analysis of the bacterial DNA isolated from mouse feces allowed us to validate our protocol and testing strategy for future mouse experiments.