Measuring Mrna Expression of LARP6 and Collagens

Document Type

Student Presentation

Presentation Date



College of Arts and Sciences


Department of Chemistry & Biochemistry

Faculty Sponsor

Dr. Lisa Warner


LARP6 (La related protein 6) is an mRNA binding protein that is linked with increased expression of Collagen type 1a1. Studies have shown that there are high levels of LARP6 mRNA in the cerebral cortex with a corresponding low mRNA profile for collagen type 1a1. It is unknown what specific function of LARP6 in the brain is. We are interested in examining the rat pheochromocytoma cell line (PC12 cells) as a model system to understand the role of LAPR6 in neuronal cells. PC12 cells can be differentiated to a neuronal phenotype by addition of nerve growth factor to the cell culture medium. PC12 cells are readily available, and relatively easy to work with. Here, we use Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) to study the mRNA expression profiles of LARP6 and collagen mRNAs that are known to interact with the LARP6 protein. Our results show that LARP6, collagen1a1, collagen2a2, and collagen5a2 are present in PC12 cells., and that gene expression of each increase.

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