Title

Synchronization of Stress-Strain Data for Analyzation of 3D Cellular Constructs Using a Biaxial Bioreactor

Document Type

Student Presentation

Presentation Date

4-15-2019

College

College of Engineering

Department

Department of Mechanical & Biomedical Engineering

Faculty Sponsor

Dr. Trevor Lujan

Abstract

Remodeling, growth, and repair of musculoskeletal soft tissues are influenced by mechanical loading conditions. To investigate this phenomenon, 3D cellular constructs can be mechanically stimulated in bioreactors and changes in mechanical behavior can be measured. However, a challenge to accurately measuring this is that applied stress and local strain must be synchronized, which has not previously been done for 3D constructs. A potential solution for the visualization and analyzation of load displacement behavior involves synchronization of 2D Digital Image Correlation (DIC) data to specific bioreactor loading cycles. In this study, a bioreactor stand was built for easy and repeatable camera set up for filming underneath the sample. Arduino technology with an LED were implemented with a custom LabVIEW program to synchronize DIC images with bioreactor loading cycles. The LED would only turn on for a specified cycle, allowing for the analysis of full-field surface strains by DIC during that specific cycle. The load cell data from LabVIEW was then taken for the specified cycle to calculate the stress, and accurate stress-strain curves were created to measure the mechanical properties of three constructs. This study developed a novel method to measure the complex stress-strain behavior of soft tissues stimulated in bioreactors.

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