Title

Developing a Differential Scanning Fluorimetry Assay to Measure Protein Stability In vitro

Document Type

Student Presentation

Presentation Date

4-16-2018

College

College of Arts and Sciences

Department

Biological Sciences

Faculty Sponsor

Lisa Warner

Abstract

Protein are important biological polymers that dictate numerous functions within various organisms. To study these important macromolecules, researchers often need to find optimal solution conditions where the protein is stable at high concentration and does not aggregate or degrade. The main objective of this project is to optimize differential scanning fluorimetry (DSF) conditions for use at Boise State. This method was pioneered in the 1950s by 1,2. Here, we are optimizing the method to help find the optimal buffer conditions for structural biology applications including solution Nuclear Magnetic Resonance (NMR) and Small Angle X-ray Scattering (SAXS). In the presented data, differential scanning fluorimetry assay, the SYPRO Orange dye was used as a readout for melting temperature. Changing the buffer conditions to increase the melting temperature of the protein indicated more stability.

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