Publication Date

12-2025

Date of Final Oral Examination (Defense)

7-8-2025

Type of Culminating Activity

Thesis

Degree Title

Master of Science in Mechanical Engineering

Department

Mechanical and Biomedical Engineering

Supervisory Committee Chair

Sophia Theodossiou, Ph.D.

Supervisory Committee Member

David Estrada, Ph.D.

Supervisory Committee Member

Zhangxian Deng, Ph.D.

Abstract

3D printing has become an indispensable tool in research labs across the globe. Yet, when it comes to bioprinting with custom or sensitive bioinks, accessible options remain limited. Commercial systems are often prohibitively expensive and restricted to proprietary materials, while DIY setups frequently lack the precision and compatibility needed for biologically relevant printing. Moreover, tunable bioinks that align with real-world biological constraints are still hard to come by. To address these gaps, we developed a modular, low-cost bioprinting platform tailored for tyramine-substituted silk fibroin (TA-SF) hydrogels-biocompatible materials known for their rapid gelation, tunable stiffness, and potential to support cell differentiation in tissue engineering applications.

Our custom-built system integrates open-source hardware, custom G-code control, and an in-line static mixer to give researchers greater control over both the printing process and material conditions. We’ve prototyped three different printers-each offering unique capabilities, from single-and dual-syringe setups to a CoreYX-based system housed within a biosafety enclosure. These designs allow us to explore a range of printing strategies under controlled conditions.

To evaluate the impact of printing on hydrogel performance, we conducted a series of experiments comparing printed constructs with traditionally hand-cast samples. Using tools like dynamic mechanical analysis (DMA), MTT assay, and live/dead cell staining, we assessed mechanical properties and biological viability. We also performed shearing analysis to better understand extrusion dynamics and their influence on material and cellular integrity. The platform has shown strong potential in making bioprinting more adaptable, reliable, and accessible for biomedical research.

Looking ahead, we aim to refine multi-layered printing during gelation, evaluate long-term hydrogel stability, and deepen our biological analysis. This project is more than just a machine-it’s a hopeful step toward unlocking the full potential of customized bioprinting for regenerative medicine and materials research.

DOI

https://doi.org/10.18122/td.2444.boisestate

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