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Publication Date
8-2021
Date of Final Oral Examination (Defense)
6-23-2021
Type of Culminating Activity
Thesis - Boise State University Access Only
Degree Title
Master of Science in Biomolecular Sciences
Department
Biology
Major Advisor
Lisa Warner, Ph.D.
Advisor
Julia Oxford, Ph.D.
Advisor
Brad Morrison, Ph.D.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Abstract
La-related protein 6 (LARP6) is an RNA binding protein with an intriguing set of functions and interactions. It has an unknown function in the brain that should be investigated further. To do this, we propose to investigate PC12 cells as a model system for studying LARP6 in the brain. PC12 cells have a long history of being used in neurodegenerative models and pharmaceutical testing due to their ability to differentiate into neuronal-like cells. We hypothesize that PC12 cells will make a good model system for studying LARP6 in the brain. In this study, we conducted western blot, PCR, immunofluorescence, IP-MS, and a morpholino knockdown of LARP6 in PC12 cells to assess our hypothesis. We concluded from our results that PC12 cells do not make a good model system for the brain.
DOI
https://doi.org/10.18122/td.1853.boisestate
Recommended Citation
Rogers, Beth, "PC12 Cells as a Model System to Study LARP6 in the Brain" (2021). Boise State University Theses and Dissertations. 1853.
https://doi.org/10.18122/td.1853.boisestate