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Publication Date
12-2019
Date of Final Oral Examination (Defense)
10-29-2019
Type of Culminating Activity
Dissertation - Boise State University Access Only
Degree Title
Doctor of Philosophy in Biomolecular Sciences
Department
Biology
Major Advisor
Eric J. Hayden, Ph.D.
Advisor
Matthew Ferguson, Ph.D.
Advisor
Adam Colson, Ph.D.
Abstract
Fluorogenic aptamers are emerging as useful molecular tools to track RNA molecules in cells and as a platform for fluorescent biosensors. The recently developed RNA Mango-I fluorogenic aptamer has a promising combination of tight binding and bright signal. However, the structure of RNA Mango limits its ability to be used for biosensor engineering. We have developed a flexible design platform for RNA Mango I that has been used to develop a novel biosensor. This process can be utilized for continual adaptation of the RNA Mango I platform into numerous new biosensors.
DOI
10.18122/td/1627/boisestate
Recommended Citation
Burden, Steven J., "The Development of Nucleic Acid Biosensors with Allosteric Fluorescence Signals" (2019). Boise State University Theses and Dissertations. 1627.
10.18122/td/1627/boisestate