Mouse Oncostatin M Transgene Construction

Publication Date

10-1-2000

Type of Culminating Activity

Thesis

Degree Title

Master of Science in Biology

Department

Biology

Supervisory Committee Chair

Cheryl Jorcyk

Supervisory Committee Member

Michael J. Spencer

Supervisory Committee Member

Richard D. Olson

Supervisory Committee Member

Marcelo Serpe

Abstract

Oncostatin M (OSM) was initially identified as a 28KD polypeptide cytokine which has been shown to inhibit the in vitro growth of cells from melanoma and other solid tumors, such as breast, ovarian, lung, cerebral meningioma, as well as normal and malignant mammary epithelial cells. While most studies using this cytokine have been performed in vitro, little is known about its in vivo biological functions and regulation of cancer cells. This thesis project involves the construction of two different mouse oncostatin M transgenes utilizing a HNP (human neutrophil peptides)-defensin-1 promoter. These transgenes (foreign genes) will be used to establish oncostatin M transgenic mice. HNP-defensin-1 promoter directs mouse Oncostatin M (mOSM) gene expression in polymorphonuclear neutrophils (PMN). These mice will be used for two long-term goals: first, to study the normal function of OSM in vivo and second, by breading (sic) these mice to another transgenic mouse line that spontaneously develops mammary tumors, to determine the role of OSM in cancer development and progression.

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