Document Type
Article
Publication Date
5-2022
Abstract
The unassisted transport of inorganic ions through lipid membranes has become increasingly relevant to an expansive range of biological phenomena. Recent simulations indicate a strong influence of a lipid membrane’s curvature on its permeability, which may be part of the overall cell sensitivity to mechanical stimulation. However, most ionic permeability experiments employ a flat, uncurved lipid membrane, which disregards the physiological relevance of curvature on such investigations. To fill this gap in our knowledge, we adapted a traditional experimental system consisting of a planar lipid membrane, which we exposed to a controlled, differential hydrostatic pressure. Our electrophysiology experiments indicate a strong correlation between the changes in membrane geometry elicited by the application of pressure, as inferred from capacitance measurements, and the resulting conductance. Our experiments also confirmed the well-established influence of cholesterol addition to lipid membranes in adjusting their mechanical properties and overall permeability. Therefore, the proposed experimental system may prove useful for a better understanding of the intricate connections between membrane mechanics and adjustments of cellular functionalities upon mechanical stimulation, as well as for confirmation of predictions made by simulations and theoretical modeling.
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Publication Information
Whiting, Rose; Finn, Pangaea W.; Bogard, Andrew; McKinney, Fulton; Pankratz, Dallin; Smith, Aviana R.; Gardner, Elen A.; and Fologea, Daniel. (2022). "Experimental Investigations on the Conductance of Lipid Membranes Under Differential Hydrostatic Pressure". Membranes, 12(5), 479. https://doi.org/10.3390/membranes12050479