Colon in Crisis: The Impact of Type 2 Diabetes Supernatant on Epithelial Health
Abstract
Type 2 Diabetes (T2D) significantly impacts blood glucose levels, with emerging evidence pointing to its effects on the gut microbiome and intestinal epithelium. This study investigates the impact of fecal supernatant derived from individuals with T2D on the confluence of Caco-2 cells, which serve as an in vitro model for the human colon. The goal of this is to Identify alterations in epithelial integrity between healthy Caco-2 cell monolayers and monolayers exposed to T2D microbiome supernatant. We aim to uncover how diabetic conditions influence the integrity and function of intestinal epithelial cells. Preliminary findings show that the gut microbiome of TD2 patients is different compared to healthy controls. We have found that supernatant from T2D patients alter the intestinal barrier permeability. Our findings will help further understand the role of the gut microbiome in TD2 patients.
Colon in Crisis: The Impact of Type 2 Diabetes Supernatant on Epithelial Health
Type 2 Diabetes (T2D) significantly impacts blood glucose levels, with emerging evidence pointing to its effects on the gut microbiome and intestinal epithelium. This study investigates the impact of fecal supernatant derived from individuals with T2D on the confluence of Caco-2 cells, which serve as an in vitro model for the human colon. The goal of this is to Identify alterations in epithelial integrity between healthy Caco-2 cell monolayers and monolayers exposed to T2D microbiome supernatant. We aim to uncover how diabetic conditions influence the integrity and function of intestinal epithelial cells. Preliminary findings show that the gut microbiome of TD2 patients is different compared to healthy controls. We have found that supernatant from T2D patients alter the intestinal barrier permeability. Our findings will help further understand the role of the gut microbiome in TD2 patients.