Designing a Cell Culture Chamber for the Direct Electrical Stimulation of Mouse Myoblast Cells Grown Ti3C2 MXene Films
Faculty Mentor Information
Dr. David Estrada (Mentor), Boise State University
Abstract
Two-dimensional (2D) materials have gained attention for their biocompatibility and limitless applications in regenerative medicine. Of significant interest are the conductive properties of Ti3C2Tx MXene that allow for the direct electrical stimulus (ES) of cells which enhances the tunability of cell differentiation and proliferation. Such advances can be applied to the regeneration of muscle tissue damaged by trauma or wound propagation. The traditional cell culture environment makes it difficult to supply a direct ES to cells without interference from the cell culture media - requiring the design of a cell culture device that facilitates a direct electrode connection to 2D films. This project focuses on the design of a 3D resin-printed chamber that temporarily secures to a glass slide to maintain cell growth on the Ti3C2Tx MXene film which extends outside of the chamber where it contacts the electrodes that supply the direct ES. Our design incorporates a new method of stability, improving the efficiency and reproducibility of experiments by replacing any need for adhesives and allowing for sample characterization without any damaging effects.
Designing a Cell Culture Chamber for the Direct Electrical Stimulation of Mouse Myoblast Cells Grown Ti3C2 MXene Films
Two-dimensional (2D) materials have gained attention for their biocompatibility and limitless applications in regenerative medicine. Of significant interest are the conductive properties of Ti3C2Tx MXene that allow for the direct electrical stimulus (ES) of cells which enhances the tunability of cell differentiation and proliferation. Such advances can be applied to the regeneration of muscle tissue damaged by trauma or wound propagation. The traditional cell culture environment makes it difficult to supply a direct ES to cells without interference from the cell culture media - requiring the design of a cell culture device that facilitates a direct electrode connection to 2D films. This project focuses on the design of a 3D resin-printed chamber that temporarily secures to a glass slide to maintain cell growth on the Ti3C2Tx MXene film which extends outside of the chamber where it contacts the electrodes that supply the direct ES. Our design incorporates a new method of stability, improving the efficiency and reproducibility of experiments by replacing any need for adhesives and allowing for sample characterization without any damaging effects.