Sequencing and Locating Double Stranded RNAs in Euglena mutabilis
Additional Funding Sources
The project described was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grant No. P20GM103408. Additional support was received from the Center of Excellence in Biomedical Research through the Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grant Nos. P20GM109095 and P20GM103408, the National Science Foundation S-STEM Gateway Scholarships in Biological Sciences under Grant Award No. DUE-1644233, and the NASA Idaho Space Grant Consortium.
Abstract
Leishmania and Trypanosoma are pathogenic protozoans that cause Leishmaniasis, Chagas disease, and sleeping sickness. These diseases infect millions of people and animals every year. It is also known that the presence of double stranded RNA (dsRNA) correlates with increased pathogenicity of Protozoa. In recent years, dsRNA have been found in Euglena mutabilis that is a non-pathogenic protozoan that is a close relative to Leishmania and Trypanosoma. By studying dsRNA found in Euglena mutabilis (Em), it could give us a better insight to the pathogenicity of Leishmania and Trypanosoma. Phenol-Chloroform extraction was used to separate dsRNA from the rest of the nucleic acid. Next Generation sequencing, cellulose chromatography and dsRNA cloning were used to sequence dsRNA. Cell fractionation was used to separate different parts of the cell, which makes it possible to identify the subcellular distribution of dsRNA.
Sequencing and Locating Double Stranded RNAs in Euglena mutabilis
Leishmania and Trypanosoma are pathogenic protozoans that cause Leishmaniasis, Chagas disease, and sleeping sickness. These diseases infect millions of people and animals every year. It is also known that the presence of double stranded RNA (dsRNA) correlates with increased pathogenicity of Protozoa. In recent years, dsRNA have been found in Euglena mutabilis that is a non-pathogenic protozoan that is a close relative to Leishmania and Trypanosoma. By studying dsRNA found in Euglena mutabilis (Em), it could give us a better insight to the pathogenicity of Leishmania and Trypanosoma. Phenol-Chloroform extraction was used to separate dsRNA from the rest of the nucleic acid. Next Generation sequencing, cellulose chromatography and dsRNA cloning were used to sequence dsRNA. Cell fractionation was used to separate different parts of the cell, which makes it possible to identify the subcellular distribution of dsRNA.