Assessing Gut Transport of Methionine and Lysine in Fast-Growing Rainbow Trout
Additional Funding Sources
The project described was supported by a student grant from the UI Office of Undergraduate Research.
Abstract
Future growth of aquaculture depends on the discovery of alternative sources of protein to feed fish. Plant-derived proteins lack sufficient levels of two essential amino acids, methionine and lysine, for fish dietary requirements. We examined differences between a genetically selected line of rainbow trout that grows more rapidly on a plant protein diet when compared to a non-selected line. It is hypothesized that genetically selected rainbow trout uptake these two limiting amino acids more efficiently in their gut via more numerous amino acid transporters as compared to non-selected fish. Amino acid uptake and total amino acids were assessed over time in plasma using [13C] and [15N] stable isotopes of methionine and lysine measured by LCMS. Amino acid transporter expression in the gut was assessed using qPCR. Results show amino acid uptake in rainbow trout selected for growth on a plant-protein diet is greater than the uptake observed in non-selected fish. Quantitative PCR of amino acid transporters in the proximal intestine also show various levels of expression over time consistent with levels of amino acid of uptake.
Assessing Gut Transport of Methionine and Lysine in Fast-Growing Rainbow Trout
Future growth of aquaculture depends on the discovery of alternative sources of protein to feed fish. Plant-derived proteins lack sufficient levels of two essential amino acids, methionine and lysine, for fish dietary requirements. We examined differences between a genetically selected line of rainbow trout that grows more rapidly on a plant protein diet when compared to a non-selected line. It is hypothesized that genetically selected rainbow trout uptake these two limiting amino acids more efficiently in their gut via more numerous amino acid transporters as compared to non-selected fish. Amino acid uptake and total amino acids were assessed over time in plasma using [13C] and [15N] stable isotopes of methionine and lysine measured by LCMS. Amino acid transporter expression in the gut was assessed using qPCR. Results show amino acid uptake in rainbow trout selected for growth on a plant-protein diet is greater than the uptake observed in non-selected fish. Quantitative PCR of amino acid transporters in the proximal intestine also show various levels of expression over time consistent with levels of amino acid of uptake.
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