Stealth Liposomes Drug Delivery and Localized Therapy
Additional Funding Sources
The project described was supported by the Pacific Northwest Louis Stokes Alliance for Minority Participation through the National Science Foundation under Award No. HRD-1410465.
Abstract
Improving cancer treatment is a hot topic of research because of the harsh effects standard chemotherapy can have on the body. Liposome drug carriers provide an improvement over standard treatment by preserving antineoplastic agents and protecting the organism from unnecessary side effects introduced by systemic application of chemotherapy. Liposomes are spherical lipid bilayer membranes that may provide localized therapy by accumulating in tumor regions. Stealth liposomes in particular are being strategically used because they have the added benefit of evading immune system detection upon introduction into the body. Both stealth and non-stealth liposomes can be loaded with a particular drug, passively or actively. Passive loading involves the formation of liposomes in solutions identical to a final desired internal composition. Active loading involves actively pushing a payload after formation of liposomes by using electrochemical gradients. In this report we will discuss preparation of liposomes using extrusion and sonication, as well as physical and functional characterization using DLS, microscopy, and spectrofluorometry.
Stealth Liposomes Drug Delivery and Localized Therapy
Improving cancer treatment is a hot topic of research because of the harsh effects standard chemotherapy can have on the body. Liposome drug carriers provide an improvement over standard treatment by preserving antineoplastic agents and protecting the organism from unnecessary side effects introduced by systemic application of chemotherapy. Liposomes are spherical lipid bilayer membranes that may provide localized therapy by accumulating in tumor regions. Stealth liposomes in particular are being strategically used because they have the added benefit of evading immune system detection upon introduction into the body. Both stealth and non-stealth liposomes can be loaded with a particular drug, passively or actively. Passive loading involves the formation of liposomes in solutions identical to a final desired internal composition. Active loading involves actively pushing a payload after formation of liposomes by using electrochemical gradients. In this report we will discuss preparation of liposomes using extrusion and sonication, as well as physical and functional characterization using DLS, microscopy, and spectrofluorometry.
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