Abstract Title
Engineering Nanocarriers for Effective Gene Delivery in T Cells
Additional Funding Sources
The project described was supported by a student grant from the UI Office of Undergraduate Research.
Abstract
With the rapid development of nanotechnology in the recent decade, novel gene delivery in T cells is being studied to replace the expensive viral vectors in immunotherapy. In this study, calcium-alginate nanoparticles were synthesized with water-in-oil emulsion method using a tip-sonicator. The obtained size and morphology of the nanoparticles were observed to be varied with volume and concentration of sodium alginate, and surfactant used. To examine the potency of Ca‐alginate nanoparticles as carriers for gene delivery in Jurkat T cells, GFP‐encoding plasmids were encapsulated in these nanoparticles to investigate transfection rate in Jurkat T cells. Our results showed that Ca‐alginate nanoparticles with an average size of 200 nm in diameter are capable for delivering gene to Jurkat T cells.
Engineering Nanocarriers for Effective Gene Delivery in T Cells
With the rapid development of nanotechnology in the recent decade, novel gene delivery in T cells is being studied to replace the expensive viral vectors in immunotherapy. In this study, calcium-alginate nanoparticles were synthesized with water-in-oil emulsion method using a tip-sonicator. The obtained size and morphology of the nanoparticles were observed to be varied with volume and concentration of sodium alginate, and surfactant used. To examine the potency of Ca‐alginate nanoparticles as carriers for gene delivery in Jurkat T cells, GFP‐encoding plasmids were encapsulated in these nanoparticles to investigate transfection rate in Jurkat T cells. Our results showed that Ca‐alginate nanoparticles with an average size of 200 nm in diameter are capable for delivering gene to Jurkat T cells.
Comments
W66