Differentiation of PC12 Cells with Nerve Growth Factor Increases the Production of LARP6 and Collagen Type 1

Additional Funding Sources

The project described was supported by Institutional Development Awards (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grant Nos. P20GM103408 and P20GM109095. We also acknowledge support from The Biomolecular Research Center at Boise State with funding from the National Science Foundation, Grant Nos. 0619793 and 0923535, the MJ Murdock Charitable Trust and the Idaho State Board of Education.

Presentation Date

7-2019

Abstract

LARP6 is a protein that is involved in the production of type 1 collagen. Collagen contributes to the severity of many fibrotic diseases, including liver fibrosis and pulmonary fibrosis. Understanding how LARP6 regulates this collagen production is important when looking for drugs to treat these diseases. Interestingly, LARP6 mRNA and protein are enriched in brain tissue. Rat pheochromocytoma cell line (PC12), which grow dendrite-like structures in the presence of NGF, was used as a model system for studying LARP6 in the brain. Immunofluorescence confocal microscopy was used to determine the localization of LARP6 and collagen in the NGF treated vs. untreated PC12 cells. In addition, RT-PCR was used to determine the levels of collagen and LARP6 mRNA expressed in NGF treated vs. untreated PC12 cells.

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Differentiation of PC12 Cells with Nerve Growth Factor Increases the Production of LARP6 and Collagen Type 1

LARP6 is a protein that is involved in the production of type 1 collagen. Collagen contributes to the severity of many fibrotic diseases, including liver fibrosis and pulmonary fibrosis. Understanding how LARP6 regulates this collagen production is important when looking for drugs to treat these diseases. Interestingly, LARP6 mRNA and protein are enriched in brain tissue. Rat pheochromocytoma cell line (PC12), which grow dendrite-like structures in the presence of NGF, was used as a model system for studying LARP6 in the brain. Immunofluorescence confocal microscopy was used to determine the localization of LARP6 and collagen in the NGF treated vs. untreated PC12 cells. In addition, RT-PCR was used to determine the levels of collagen and LARP6 mRNA expressed in NGF treated vs. untreated PC12 cells.