Effects of Lamin A/C Depletion on Nuclear Structure and LINC Complex

Additional Funding Sources

This project is supported by a 2017-2018 STEM Undergraduate Research Grant from the Higher Education Research Council.

Presentation Date

7-2018

Abstract

It is hypothesized that silencing the lamin A/C gene, LMNA, will caused deformation in the nucleus of a mammalian cell. In order to test this, we transfected Mesenchymal Stem Cells with an siRNA transfection reagent and used fluorescence imaging to analyze the results. The results showed that silencing LMNA affects both Nesprin 1 and Sun 1 proteins, which are part of the LINC complex, and changes nuclear shape.

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Effects of Lamin A/C Depletion on Nuclear Structure and LINC Complex

It is hypothesized that silencing the lamin A/C gene, LMNA, will caused deformation in the nucleus of a mammalian cell. In order to test this, we transfected Mesenchymal Stem Cells with an siRNA transfection reagent and used fluorescence imaging to analyze the results. The results showed that silencing LMNA affects both Nesprin 1 and Sun 1 proteins, which are part of the LINC complex, and changes nuclear shape.