Abstract Title
The Role of Low Intensity Vibrations in Adipocyte Inflammatory Response
Additional Funding Sources
The project described was supported by the Pacific Northwest Louis Stokes Alliance for Minority Participation through the National Science Foundation under Award No. HRD-1410465. We also acknowledge support from The Biomolecular Research Center at Boise State with funding from an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grants No. P20GM103408 and P20GM109095, the National Science Foundation Grant Nos. 0619793 and 0923535, the MJ Murdock Charitable Trust, and the Idaho State Board of Education.
Abstract
Adipocytes found in bone marrow and adipose tissues functions an energy depot for excess energy. Excessive lipid storage in adipocytes in cases like obesity leads to secretion of pro-inflammatory cytokines, causing chronic inflammation associated with type II diabetes and other metabolic disorders. Low intensity vibration (LIV) – an exercise mimetic – decreases fat accumulation in bone marrow and adipose tissues. While we have shown that LIV suppression of adipogenesis relies on activation of F-actin regulatory signaling pathways, the role of LIV in adipocyte inflammatory cytokine secretion is unknown.Using adipocyte progenitor mesenchymal stem cells (MSC), the goal of this study is to understand whether LIV can lower the inflammatory marker secretion of adipocytes. We hypothesize that the LIV sustains higher F-actin levels during MSC differentiation to adipocytes and decrease secretion of pro-inflammatory signaling molecule monocyte chemoattractant protein-1 (MCP-1). To test this hypothesis MSCs will be subjected to adipogenic differentiation media for five days. During differentiation MSCs will be subjected to LIV (90Hz, 0.7g) twice daily for 20 minutes, MCP-1 expression and protein levels will be measured. An additional group will be used to measure F-actin levels via immunostaining. Findings will reveal if LIV can decrease inflammation in adipose tissues.
The Role of Low Intensity Vibrations in Adipocyte Inflammatory Response
Adipocytes found in bone marrow and adipose tissues functions an energy depot for excess energy. Excessive lipid storage in adipocytes in cases like obesity leads to secretion of pro-inflammatory cytokines, causing chronic inflammation associated with type II diabetes and other metabolic disorders. Low intensity vibration (LIV) – an exercise mimetic – decreases fat accumulation in bone marrow and adipose tissues. While we have shown that LIV suppression of adipogenesis relies on activation of F-actin regulatory signaling pathways, the role of LIV in adipocyte inflammatory cytokine secretion is unknown.Using adipocyte progenitor mesenchymal stem cells (MSC), the goal of this study is to understand whether LIV can lower the inflammatory marker secretion of adipocytes. We hypothesize that the LIV sustains higher F-actin levels during MSC differentiation to adipocytes and decrease secretion of pro-inflammatory signaling molecule monocyte chemoattractant protein-1 (MCP-1). To test this hypothesis MSCs will be subjected to adipogenic differentiation media for five days. During differentiation MSCs will be subjected to LIV (90Hz, 0.7g) twice daily for 20 minutes, MCP-1 expression and protein levels will be measured. An additional group will be used to measure F-actin levels via immunostaining. Findings will reveal if LIV can decrease inflammation in adipose tissues.
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