Viral Co-infection of Mouse Lung Epithelial Cells
Additional Funding Sources
The project described was supported by the Research Experience for Undergraduates Program Site: Molecular and organismal evolution at the University of Idaho under Award No. 1757826.
Presentation Date
7-2018
Abstract
Viral Co-infection of Mouse Lung Epithelial Cells
Patients hospitalized with respiratory infections have often been shown to be infected with multiple viruses. Studies with co-infected mice have shown there is a difference in infection severity if the mice are singly or co-infected. Little information exists on how two different viruses interact in shared cell population, and whether an immune response or competitive inhibition limits growth of one of the viruses. This study observes differences in viral titer from lung epithelial cell populations infected by a single virus or co-infected by murine coronavirus and rhinovirus. The supernatant medium was collected every 6 hours post-infection for up to 48 hours. To determine viral titers at each timepoint, a 50% tissue culture infective dose (TCID50 Assay) was used. Comparing viral titers between multiple time points will give insight into the difference between the viral growth of co-infection against single infection could be determined. These data will provide insight into how co-infections behave within a cell population.
1Andres Rodriguez, 1Andres Gonzales, 1Tanya Miura
Department of Biological Sciences and Center for Modeling Complex Interactions, University of Idaho1, Moscow, ID
"This project was funded in part by the National Science Foundation REU Site award No. 1757826"
Viral Co-infection of Mouse Lung Epithelial Cells
Viral Co-infection of Mouse Lung Epithelial Cells
Patients hospitalized with respiratory infections have often been shown to be infected with multiple viruses. Studies with co-infected mice have shown there is a difference in infection severity if the mice are singly or co-infected. Little information exists on how two different viruses interact in shared cell population, and whether an immune response or competitive inhibition limits growth of one of the viruses. This study observes differences in viral titer from lung epithelial cell populations infected by a single virus or co-infected by murine coronavirus and rhinovirus. The supernatant medium was collected every 6 hours post-infection for up to 48 hours. To determine viral titers at each timepoint, a 50% tissue culture infective dose (TCID50 Assay) was used. Comparing viral titers between multiple time points will give insight into the difference between the viral growth of co-infection against single infection could be determined. These data will provide insight into how co-infections behave within a cell population.
1Andres Rodriguez, 1Andres Gonzales, 1Tanya Miura
Department of Biological Sciences and Center for Modeling Complex Interactions, University of Idaho1, Moscow, ID
"This project was funded in part by the National Science Foundation REU Site award No. 1757826"
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