Cytokine-induced microRNA Expression in Breast and Prostate Cancer
Abstract
Inflammatory cytokines have in recent years been tied to the process of tumor progression, invasion, and metastasis. In particular, cytokines are known to influence the expression of microRNAs (miRNAs) that regulate cancer. miRNAs are small noncoding RNAs, which function by post-transcriptional silencing of gene expression. This project investigates miRNAs regulated by inflammatory cytokines in both human breast and prostate cancer cell lines. Human T47D breast and DU-145 prostate cancer cells were treated with or without inflammatory cytokines (25 ng/ml) for 24 hours. RNA was collected and analyzed on miRNA microarrays. Inflammatory cytokine up- or down-regulation of miRNAs in the cancer cells is currently being confirmed by reverse transcription-quantitative polymerase chain reaction (qRT-PCR). This project has potential to result in novel treatment options for cancer, which is one of the leading causes of death in developed countries.
Cytokine-induced microRNA Expression in Breast and Prostate Cancer
Inflammatory cytokines have in recent years been tied to the process of tumor progression, invasion, and metastasis. In particular, cytokines are known to influence the expression of microRNAs (miRNAs) that regulate cancer. miRNAs are small noncoding RNAs, which function by post-transcriptional silencing of gene expression. This project investigates miRNAs regulated by inflammatory cytokines in both human breast and prostate cancer cell lines. Human T47D breast and DU-145 prostate cancer cells were treated with or without inflammatory cytokines (25 ng/ml) for 24 hours. RNA was collected and analyzed on miRNA microarrays. Inflammatory cytokine up- or down-regulation of miRNAs in the cancer cells is currently being confirmed by reverse transcription-quantitative polymerase chain reaction (qRT-PCR). This project has potential to result in novel treatment options for cancer, which is one of the leading causes of death in developed countries.