Mammalian Cytotoxicity Study of Novel Organometallic Complexes
Ken Cornell, Ph.D.
Antibiotic resistance is becoming more and more prevalent in our society to the point that even simple diseases can be life threatening. Thus, there is a significant need for novel antibiotics that can combat these diseases and reduce future complications when a disease becomes resistant to a treatment. While there is a need for more novel antibiotics, one must also understand the nature of the compound and all of the effects that it will have on both the pathogen and the host body before it is distributed. The compounds that we are studying are novel organometallics. While there is minimal research on these compounds, there is evidence that there may be cytotoxic effects against mammalian cells. The purpose of this study was to determine the cytotoxic effects of the organometallic compounds against 2 different human cell lines using a Resazurin Cell Viability Assay. The data was analyzed using GraphPad Prism software to calculate IC50 values . In the absence of the metal ions, the organic monomer toxicity was cell line dependent, with the A549 cells much more sensitive than the K562 cells. Once metal complexes were formed, the cytotoxicy was generally increased for both cell lines, with IC50 values in the low micromolar range.
Harrison, Nathan; Leach, Caleb; Lusk, Kyle; Waynant, Kris; and Cornell, Ken, "Mammalian Cytotoxicity Study of Novel Organometallic Complexes" (2021). 2021 Undergraduate Research Showcase. 147.