Evaluation of Two Expression Vectors for Cholera Toxin CTA2/B Chimera Expression

Document Type

Student Presentation

Presentation Date



College of Arts and Sciences


Department of Biological Sciences

Faculty Sponsor

Dr. Juliette Tinker


Staphylococcus aureus infection in dairy cows can lead to a chronic, contagious disease of the utter called mastitis. The creation of an effective vaccine could reduce costs in infection treatment by dairy farmers and improve the living standard for the dairy cows while reducing antibiotic dependence. Additionally, it could improve human vaccine approaches. Cholera toxin has a potent mucosal adjuvant activity and can act as a carrier molecule; thus, it is an ideal candidate for vaccine production. CTA2/B chimeras are non-toxic fusions of cholera toxin and an antigen, and can be administered intranasally to induce lasting immunogenicity. Here we have constructed a novel vector with two promoters to express CTA2/B chimeras, and cloned a fluorescent molecule (red fluorescent protein) and a Staphylococcus aureus antigen (IsdA) into these vectors. CTA2/B chimera production will be compared to that from a second vector that expresses both peptides from one promoter (pARLDR19). These expression vectors will be used to improve S. aureus vaccine expression and purification. Intranasal S.aureus vaccine delivery is a potential cost effective, humane vaccine modality that could lead to a reduction bovine mastitis.

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