Forming a Bovine Mastitis Vaccine Using S. Aureus IsdH and Cholera Toxin
College of Arts and Sciences
Staphylococcus aureus infection in humans and animals is on the rise due to the increase of antibiotic resistant strains such as methicillin-resistant S. aureus (MRSA). Developing a vaccine will improve health and reduce the need for antibiotic use. S. aureus surface proteins are promising vaccine candidates. The IsdH adhesin is involved in both host binding and iron uptake and known to be expressed during infection. The IsdH gene was isolated from the bovine S. aureus Newbould 305 strain using primers to promote directional cloning into a vector containing the cholera toxin cta2/ctbB genes. Next, the isolated gene and vector pARLDR19 were digested and purified using SphI and ClaI enzymes. isdH was then ligated into the plasmid and transformed into Escherichia coli. To ensure the correct plasmid was transformed, a polymerase chain reaction (PCR) was completed with isdH primers and positive and negative controls. The protein will be expressed from this plasmid and purified for testing in future studies to detect immunogenicity in bovines.
Truman, Nateijie, "Forming a Bovine Mastitis Vaccine Using S. Aureus IsdH and Cholera Toxin" (2018). 2018 Undergraduate Research and Scholarship Conference. 99.