Nuclear Response to Low Intensity Vibrations
College of Engineering
Mechanical and Biomedical Engineering
The nucleus, central to all cellular activity, relies on both direct mechanical input as well as its molecular transducers to sense external stimuli and respond by regulating intra-nuclear organization that ultimately determines gene expression to control cell function and fate. It is long studied that signals propagate from extracellular environment to cytoskeleton and into the nucleus (outside-in signaling) to regulate cell behavior. Emerging evidence, however, has shown that both the cytoskeleton and nucleus have an inherent ability to sense and adapt to mechanical force independent of each other. This suggests mechano-signaling and cytoskeleton remodeling events in response to exercise mimetics, like low intensity vibration (LIV), may directly be sensed at the nucleus (inside-out signaling). Here we hypothesize that cell nuclei will directly adapt to dynamic accelerations in response to LIV. To answer this question directly, we isolated live nuclei from cells to test their mechanical responses to LIV. Isolated nuclei are introduced to a low intensity vibration (LIV, 0.7g, 90Hz) and their stiffness’ measured via AFM (Atomic force microscope) protocol. Findings from this study will allow evaluation of the mechanical role that the nucleus plays in the cell as an individual organelle.
Newberg, Josh; Schimpf, Jesse; and Davis, Paul H., "Nuclear Response to Low Intensity Vibrations" (2018). 2018 Undergraduate Research and Scholarship Conference. 49.