Isotyping and Purification of Antibodies for Therapeutic and Diagnostic Use

Document Type

Student Presentation

Presentation Date



College of Arts and Sciences


Biological Sciences

Faculty Sponsor

Julia Oxford


Collagen is the most abundant protein in the body and major consequences result if it does not function properly. Collagen IX (Col 9) is a component of the cartilage extracellular matrix. Diseases such as osteoarthritis, degenerative disc disorders and chondrodysplasias are associated with mutations in Col 9. Monoclonal antibodies are a novel advance in medicine, as they can be used to diagnose and treat disorders by binding to specific antigens. To find new diagnostic and therapeutic methods in treating Col 9 disorders, a monoclonal antibody was created in our laboratory. Isotype characterization, species specificity, immunocytochemistry, and western blot compatibility will be determined so that the antibodies can be used for further research.

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