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Publication Date

8-13-2021

Date of Final Oral Examination (Defense)

June 2021

Type of Culminating Activity

Thesis - Boise State University Access Only

Degree Title

Master of Science in Biomolecular Sciences

Department

Biology

Major Advisor

Lisa Warner, Ph.D.

Advisor

Julia Oxford, Ph.D.

Advisor

Brad Morrison, Ph.D.

Creative Commons License

Creative Commons Attribution-NonCommercial 4.0 International License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

Abstract

La-related protein 6 (LARP6) is an RNA binding protein with an intriguing set of functions and interactions. It has an unknown function in the brain that should be investigated further. To do this, we propose to investigate PC12 cells as a model system for studying LARP6 in the brain. PC12 cells have a long history of being used in neurodegenerative models and pharmaceutical testing due to their ability to differentiate into neuronal-like cells. We hypothesize that PC12 cells will make a good model system for studying LARP6 in the brain. In this study, we conducted western blot, PCR, immunofluorescence, IP-MS, and a morpholino knockdown of LARP6 in PC12 cells to assess our hypothesis. We concluded from our results that PC12 cells do not make a good model system for the brain.

DOI

https://10.18122/td.1853.boisestate

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