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Document Type

Abstract

Publication Date

1-14-2026

Abstract

Up-regulation of KHK activation on a high fructose diet is positively associated with the subsequent development of several metabolic diseases, including high fructose diet-induced heart disease. Hypoxic exercise is considered an effective means of treating and preventing metabolic diseases and improving cardiac function. However, it is unclear whether the link between hypoxic exercise and high fructose-induced heart disease exists. Therefore, the aim of this study was to investigate the link between the modulation of KHK by hypoxic exercise and high fructose diet heart disease. Method: Three hundred virgin flies of the wild-type W1118 strain were collected and randomly assigned to control (NC), high fructose diet (HF), and high fructose diet (HE) groups, with 100 flies’ group. Drosophila on a high fructose diet were fed continuously with high fructose from birth to 12 days old, and Drosophila on hypoxic exercise were fed with a self-developed hypoxic exercise device for 5 consecutive days of 1 hour per day from 7 days old, and each group was sampled at 13 days old. Changes in KHK expression levels in the heart were detected by RT-qPCR, and various kinds of Drosophila cardiac function were quantified using an EM-CCD digital camera and semi-automatic optical heartbeat analysis software. Compared with the NC group, KHK mRNA expression was increased (P < 0.05), arrhythmia index was increased (P < 0.05), systolic intervals (P < 0.01), diastolic intervals were bradycardic, systolic diameter and diastolic diameter were decreased (P < 0.01), and ejection fraction was decreased (P < 0.001) in Drosophila hearts after the high fructose diet; compared with the HF group, in Drosophila hearts of the HE group KHK mRNA expression decreased (P < 0.05), arrhythmia index decreased (P < 0.05), cardiac inter-systolic interval decreased (P < 0.001), diastolic bradycardia improved, systolic diameter increased, diastolic diameter increased (P < 0.05), and ejection fraction increased (P < 0.01) in Drosophila hearts from the HE group. Hypoxic exercise improved cardiac rhythmic function and pumping dysfunction on a high-fructose diet by inhibiting KHK expression in the heart.

DOI

https://doi.org/10.18122/ijpah.5.1.118.boisestate

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