Abstract Title
Assessing the Effects of Hydrogen Bonding on the Reactivity of Zinc Thiolates
Additional Funding Sources
This project is supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award No. R25GM123927. Boise State University
Abstract
Methylated DNA has been proposed as a possible cause of cancer due to transcription errors. Organisms have developed ways of correcting methylated DNA, in prokaryotes the Ada Repair Protein is responsible for correcting those errors. The Ada Repair Protein is a zinc-containing metalloprotein coordinated to four cysteine residues. It is proposed that Cys-38 reacts with the methylated DNA, but the mechanism by which this reaction occurs is not solidified. It is also unknown whether hydrogen bonding has an effect on the reactivity of the bound thiolates.
Assessing the Effects of Hydrogen Bonding on the Reactivity of Zinc Thiolates
Methylated DNA has been proposed as a possible cause of cancer due to transcription errors. Organisms have developed ways of correcting methylated DNA, in prokaryotes the Ada Repair Protein is responsible for correcting those errors. The Ada Repair Protein is a zinc-containing metalloprotein coordinated to four cysteine residues. It is proposed that Cys-38 reacts with the methylated DNA, but the mechanism by which this reaction occurs is not solidified. It is also unknown whether hydrogen bonding has an effect on the reactivity of the bound thiolates.