Title of Submission
YAP Mediated Interaction of Simulated Microgravity and Irradiation in Mesenchymal Stem Cells
Biomolecular Sciences, MS
Major Advisor Name
Type of Submission
Space travel’s major components, radiation and microgravity, impact the musculoskeletal system. Mesenchymal stem cells (MSC), the progenitor cells of osteoblasts: key for bone formation; are critical to the maintenance of the musculoskeletal system. Previous research showed a reduction of Yap1 and MSC occurred post simulated microgravity (sMG). In PC12 and fibroblast cell types sMG induced senescence while gamma irradiation was shown to cause senescence in MSC1,2. YAP1 knockdown instigated senescence and was reduced in other γ-irradiated cell types3. We sought to study the concomitant effects of sMG and radiation on the senescence, apoptosis and YAP1 levels of bone marrow MSC.
1. Jinghua Wang, Jifei Zhang, Shasha Bai, Guangyou Wang, Lili Mu, Bo Sun, Dandan Wang, Qingfei Kong, Yumei Liu, Xiuhua Yao, Ying Xu, Hulun Li, Simulated microgravity promotes cellular senescence via oxidant stress in rat PC12 cells, Neurochemistry International, Volume 55, Issue 7, 2009, Pages 710-716, ISSN 0197-0186, https://doi.org/10.1016/j.neuint.2009.07.002.
2. Cmielova, J., Havelek, R., Soukup, T., Jiroutová, A., Visek, B., Suchánek, J., … Rezacova, M. (2012). Gamma radiation induces senescence in human adult mesenchymal stem cells from bone marrow and periodontal ligaments. International Journal of Radiation Biology, 88(5), 393–404. https://doi.org/10.3109/09553002.2012.666001
3. Zhang, L., Cheng, F., Wei, Y., Zhang, L., Guo, D., Wang, B., & Li, W. (2018). Inhibition of TAZ contributes radiation-induced senescence and growth arrest in glioma cells. Oncogene. https://doi.org/10.1038/s41388-018-0626-0
This study was supported by NASA EPSCoR NNX15AK35A, NSBRI PF04304, NASA ISGC NNX15AI04H, COBRE Matrix Biology program at Boise State University (P20GM109095) and Idaho INBRE (P20GM103408 )