2019 Graduate Student Showcase
 

Title of Submission

Transcriptional Activation by Glucocorticoid Receptor Studied by 3D Orbital Tracking Fluorescence Cross Correlation Spectroscopy

Degree Program

Biomolecular Sciences, PhD

Major Advisor Name

Matthew Ferguson

Type of Submission

Scholarly Poster

Judging Session

Both Judging Sessions

Abstract

Understanding the genome and the interactions taking place on a molecular level in living cells is difficult. But it is critical for understanding gene regulation,transcription and cellular metabolism. Through 3D orbital tracking we can visualize transcription by observing the relationship between pre-mRNA synthesis and the protein-DNA binding of the transcription factor. This study establishes that we can track these molecules of interest, fluorescently labeled pre-mRNA and the transcription factor, glucocorticoid receptor during transcriptional activation. The data collected enhances our knowledge of eukaryotic transcriptional regulation and highlights previously unknown details about transcriptional activation and temporal coordination.

Funding Information

Our research is funded by Research Corporation for Science Advancement and the Gordon and Betty Moore Foundation through Grant GBMF5263.10 and by National Institute of General Medical Sciences through Grant 1R15GM123446.

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