2019 Graduate Student Showcase
 

Title of Submission

Measuring Aptamer Affinity with Kinexa

Degree Program

Biomolecular Sciences, PhD

Major Advisor Name

Daniel Fologea

Type of Submission

Scholarly Poster

Judging Session

9am - 10:30am - Judging Session #1

Abstract

Aptamers are nucleic acid or peptide molecules selected to have a high affinity and specificity for their substrates, very often in the sub-nanomolar range. Consequently, precise and reliable determination of their binding constant to the targets using techniques traditionally based on real-time monitoring is extremely challenging. To alleviate these roadblocks, we investigated the usefulness of the Kinetics Exclusion Assay (KinExA) technology to assess the equilibrium when high-affinity aptamers are employed. Although this technology was specifically developed for characterization of antibody-antigen interactions down to the femtomolar range, we adapted it for routine measurements of interactions that employ DNA aptamers.

Funding Information

Biomolecular Sciences Graduate Programs, NSF Career grant 1554166, Idaho State Board of Education grant IF18-005

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