Insights into β-Ketoacyl-Chain Recognition for β-Ketoacyl-ACP Utilizing AHL Synthases
Beta-ketoacyl-ACP utilizing enzymes in fatty acid, polyketide and acyl-homoserine lactone biosynthetic pathways are important targets for developing antimicrobial, anticancer and antiparasitic compounds. Published reports on successful isolation of beta-ketoacyl-ACPs in a laboratory remain scarce to date and thus most beta-ketoacyl-ACP utilizing enzymes are routinely characterized using small molecule substrates in lieu of the bonafide 3-oxoacyl-ACPs. We report the systematic investigation into the electronic, geometric and spatial aspects of beta-ketoacyl-chain recognition to develop 3-oxoacyl-ACP substrate mimics for two beta-ketoacyl-ACP utilizing quorum signal synthases.
Lam, Mila Nhu; Dudekula, Dastagiri; Durham, Bri; Collingwood, Noah; Brown, Eric C.; and Nagarajan, Rajesh. (2018). "Insights into β-Ketoacyl-Chain Recognition for β-Ketoacyl-ACP Utilizing AHL Synthases". Chemical Communications, 54(64), 8838 - 8841.