Two synthetic aziridinomitosenes (AZMs), Me-AZM and H-AZM, structurally related to mitomycin C (MC) were evaluated for their anticancer activity against six cancer cell lines (HeLa, Jurkat, T47D, HepG2, HL-60, and HuT-78) and tested for their DNA-modifying abilities in Jurkat cells. Cytotoxicity assays showed that Me-AZM is up to 72-fold and 520-fold more potent than MC and H-AZM, respectively. Me-AZM also demonstrated increased DNA modification over MC and H-AZM in alkaline COMET and Hoechst fluorescence assays that measured crosslinks in cellular DNA. Me-AZM and H-AZM treatment of Jurkat cells was found to sponsor significant DNA-protein crosslinks using a K-SDS assay. The results clearly indicate that the AZM C6/C7 substitution pattern plays an important role in drug activity and supports both DNA-DNA and DNA-protein adduct formation as mechanisms for inducing cytotoxic effects.
This is an author-produced, peer-reviewed version of this article. © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. Details regarding the use of this work can be found at: https://creativecommons.org/licenses/by-nc-nd/4.0/. The final, definitive version of this document can be found online at Bioorganic & Medicinal Chemistry, doi: 10.1016/j.bmc.2015.10.028
Mallory, Chris M.; Carfi, Ryan P.; Moon, SangPhil; Cornell, Kenneth A.; and Warner, Don L.. (2015). "Modification of Cellular DNA by Synthetic Aziridinomitosenes". Bioorganic & Medicinal Chemistry, 23(23), 7378-7385. http://dx.doi.org/10.1016/j.bmc.2015.10.028