Synthesis and Characterization of Inorganic Compounds Relevant to Heterocumulene Activation by Carbonic Anhydrase
Carbonic anhydrase (CA) is a metalloenzyme of great importance that is found in all living organisms. The primary biological function of CA is the reversible hydration of carbon dioxide (CO2) to form bicarbonate; a task it performs at a high catalytic efficiency that approaches the diffusion-control limit. However, research directed at understanding the mechanism of carbonyl sulfide (COS) and carbon disulfide (CS2) activation by CA has been very limited and is the motivation for this research. Synthetic models, which mimic the active site of CA, have been synthesized and their reactivity with sulfur-containing heterocumulenes (i.e. COS and CS2) explored. Details of the reactivity and characterization of complexes relevant to intermediates in the catalytic cycle of CA will be presented.