Mouse Oncostatin M Transgene Construction
Type of Culminating Activity
Master of Science in Biology
Michael J. Spencer
Richard D. Olson
Oncostatin M (OSM) was initially identified as a 28KD polypeptide cytokine which has been shown to inhibit the in vitro growth of cells from melanoma and other solid tumors, such as breast, ovarian, lung, cerebral meningioma, as well as normal and malignant mammary epithelial cells. While most studies using this cytokine have been performed in vitro, little is known about its in vivo biological functions and regulation of cancer cells. This thesis project involves the construction of two different mouse oncostatin M transgenes utilizing a HNP (human neutrophil peptides)-defensin-1 promoter. These transgenes (foreign genes) will be used to establish oncostatin M transgenic mice. HNP-defensin-1 promoter directs mouse Oncostatin M (mOSM) gene expression in polymorphonuclear neutrophils (PMN). These mice will be used for two long-term goals: first, to study the normal function of OSM in vivo and second, by breading (sic) these mice to another transgenic mouse line that spontaneously develops mammary tumors, to determine the role of OSM in cancer development and progression.
Zhang, Yumei Lynda, "Mouse Oncostatin M Transgene Construction" (2000). Boise State University Theses and Dissertations. 1179.