We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process.
Kim, Byung I. and Boehm, Ryan. (2012). "Force-Feedback High-Speed Atomic Force Microscope for Studying Large Biological Systems". Micron, 43(12), 1372-1379. http://dx.doi.org/10.1016/j.micron.2012.04.005