Abstract Title

Deficient Low-Light Photoreactivation in an Excision-Repair Mutant of Chlamydomonas Compared To Wildtype

Disciplines

Biotechnology | Cell Biology | Genetics | Molecular Genetics

Abstract

Photoreactivation is the rescue from killing of UV-exposed cells by subsequent exposure to visible light. Unexpectedly, we find that a completely excision-repair mutant of single celled alga Chlamydomonas reinhardtii is also deficient in photoreactivation relative to wild-type at low levels of post-UV visible light treatment. However, we find that increased visible light flux is able to rescue these mutants post-UV in a dose-dependent manner. Here we present our initial characterization of this phenomenon, as well as the results to date of our investigation into its physiological basis.

Comments

Poster #Th33

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Deficient Low-Light Photoreactivation in an Excision-Repair Mutant of Chlamydomonas Compared To Wildtype

Photoreactivation is the rescue from killing of UV-exposed cells by subsequent exposure to visible light. Unexpectedly, we find that a completely excision-repair mutant of single celled alga Chlamydomonas reinhardtii is also deficient in photoreactivation relative to wild-type at low levels of post-UV visible light treatment. However, we find that increased visible light flux is able to rescue these mutants post-UV in a dose-dependent manner. Here we present our initial characterization of this phenomenon, as well as the results to date of our investigation into its physiological basis.