Utilizing DNA Barcoding and Bioinformatics to Identify Plant Species on NNU Campus
Presentation Date
7-2015
Abstract
Background: Dr Ronald Strohmeyer at NNU had his research students utilize DNA barcoding as a tool to extract DNA from different plants on campus. Then, the identified plants got labeled and a plaque was made to show their information to all who pass by the campus. DNA barcoding is a way to identify species when normal taxonomy isn’t suffice. Methods: First, different plant samples were collected from the NNU campus. Second, performed either of two protocols; Carolina and Wizard. Third, amplified the DNA by polymerase chain reaction. Fourth, analyzed the polymerase chain reaction by gel electrophoresis. And lastly, used bioinformatics to review results. Results: We collected a total of 16 plant samples, and identified 14. Conclusion: All the plants samples collected were already known species found in an online database. Plaques could now be made to identify these plants on campus for all to observe.
Utilizing DNA Barcoding and Bioinformatics to Identify Plant Species on NNU Campus
Background: Dr Ronald Strohmeyer at NNU had his research students utilize DNA barcoding as a tool to extract DNA from different plants on campus. Then, the identified plants got labeled and a plaque was made to show their information to all who pass by the campus. DNA barcoding is a way to identify species when normal taxonomy isn’t suffice. Methods: First, different plant samples were collected from the NNU campus. Second, performed either of two protocols; Carolina and Wizard. Third, amplified the DNA by polymerase chain reaction. Fourth, analyzed the polymerase chain reaction by gel electrophoresis. And lastly, used bioinformatics to review results. Results: We collected a total of 16 plant samples, and identified 14. Conclusion: All the plants samples collected were already known species found in an online database. Plaques could now be made to identify these plants on campus for all to observe.