Abstract Title

An Oxidative Pretreatment Decreases Efficiency of UV Photoreactivation in Chlamydomonas

Disciplines

Life Sciences

Abstract

Plant cells are unavoidably subject to multiple genotoxic stresses, both from external sources (e.g., solar UV, tropospheric ozone) and as a result of endogenous metabolism (e.g., photosynthesis). Human activities are altering the atmosphere in ways that may significantly alter the exposure of plant cells to such endogenous and exogenous genotoxic stressors in both agricultural and natural ecosystems. We are interested in the interaction of such stressors in plant cells, and are using the unicellular microalga Chlamydomonas reinhardtii as the model system for our research. We find that gametes of Chlamydomonas exposed to an oxidative pretreatment (acridine orange and visible light) and increased UV irradiation (200 J/m2) are highly statistically significantly deficient in UV photoreactivation (measured as colony forming units).

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An Oxidative Pretreatment Decreases Efficiency of UV Photoreactivation in Chlamydomonas

Plant cells are unavoidably subject to multiple genotoxic stresses, both from external sources (e.g., solar UV, tropospheric ozone) and as a result of endogenous metabolism (e.g., photosynthesis). Human activities are altering the atmosphere in ways that may significantly alter the exposure of plant cells to such endogenous and exogenous genotoxic stressors in both agricultural and natural ecosystems. We are interested in the interaction of such stressors in plant cells, and are using the unicellular microalga Chlamydomonas reinhardtii as the model system for our research. We find that gametes of Chlamydomonas exposed to an oxidative pretreatment (acridine orange and visible light) and increased UV irradiation (200 J/m2) are highly statistically significantly deficient in UV photoreactivation (measured as colony forming units).