Abstract Title

Assessment of Antigen Specific Humoral Immune Response in Dairy Cows After Administration of IsdA-CTA_2/B +ClfA_2/B Vaccine

Disciplines

Biology | Microbiology

Abstract

Bovine mastitis caused by Staphylococcus aureus is a crucial problem in the dairy industry. It not only causes damage to the cow, but a high somatic cell count (SCC) in the milk, which forces the farmer to dispose of the milk, costing the farmer considerable amounts of money. Many attempts at creating a vaccine for Staphylococcus aureus in both cows and humans have thus far failed. In the current study, 21 dairy cows were chosen to be assessed based on the absence of colonized Staphylococcus aureus prior to vaccine administration. Each cow was vaccinated via the nasal route with the /B chimera, composed of a Cholera toxin adjuvant covalently fused with the Staphylococcal proteins, Iron-regulated surface determinant A (IsdA) and clumping factor A (ClfA). Nasal, serum, and milk samples were taken from each cow to assess the antigen specific humoral response elicited by the vaccine. Quantitative investigation of immune response was accomplished by analyzing bovine samples for antibodies IgG and IgA against antigens Isda and ClfA via ELISA protocol, while qualitative immune response was investigated using an Immunobot (Western).

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Assessment of Antigen Specific Humoral Immune Response in Dairy Cows After Administration of IsdA-CTA_2/B +ClfA_2/B Vaccine

Bovine mastitis caused by Staphylococcus aureus is a crucial problem in the dairy industry. It not only causes damage to the cow, but a high somatic cell count (SCC) in the milk, which forces the farmer to dispose of the milk, costing the farmer considerable amounts of money. Many attempts at creating a vaccine for Staphylococcus aureus in both cows and humans have thus far failed. In the current study, 21 dairy cows were chosen to be assessed based on the absence of colonized Staphylococcus aureus prior to vaccine administration. Each cow was vaccinated via the nasal route with the /B chimera, composed of a Cholera toxin adjuvant covalently fused with the Staphylococcal proteins, Iron-regulated surface determinant A (IsdA) and clumping factor A (ClfA). Nasal, serum, and milk samples were taken from each cow to assess the antigen specific humoral response elicited by the vaccine. Quantitative investigation of immune response was accomplished by analyzing bovine samples for antibodies IgG and IgA against antigens Isda and ClfA via ELISA protocol, while qualitative immune response was investigated using an Immunobot (Western).