Microfluidic Devices Integrating Microcavity Surface-Plasmon-Resonance Sensors: Glucose Oxidase Binding-Activity Detection

Authors

Dragos Amarie, Indiana University
Abdelkrim Alileche, Boise State UniversityFollow
Bogdan Dragnea, Indiana University
James A. Glazier, Indiana University

Document Type

Article

Publication Date

1-1-2010

DOI

http://dx.doi.org/10.1021/ac902038d

Abstract

We have developed miniature (≈1 μm diameter) microcavity surface-plasmon-resonance sensors (MSPRS), integrated them with microfluidics, and tested their sensitivity to refractive-index changes. We tested their biosensing capability by distinguishing the interaction of glucose oxidase (M_r 160 kDa) with its natural substrate (β-Dglucose, Mr 180 Da) from its interactions with nonspecific substrates (L-glucose, D-mannose, and 2-deoxy-D-glucose). We ran the identical protocol we had used with the MSPRS on a Biacore 3000 instrument using their bare gold chip. Only the MSPRS was able to detect β-D-glucose binding to glucose oxidase. Each MSPRS can detect the binding to its surface of fewer than 35 000 glucose oxidase molecules (representing 9.6 fg or 60 zmol of protein), about 10⁶ times fewer than classical surface-plasmon-resonance biosensors.

Publication Information

Amarie, Dragos; Alileche, Abdelkrim; Dragnea, Bogdan; and Glazier, James A.. (2010). "Microfluidic Devices Integrating Microcavity Surface-Plasmon-Resonance Sensors: Glucose Oxidase Binding-Activity Detection". Analytical Chemistry, 82(1 (Compendex)), 343-352.