Non-specific ion conductance channels can be formed in lipid membranes by the poreforming toxin lysenin. These channels are voltage regulated and are responsive to changes in metal ion concentration. In our research, we studied the effects of metal ion concentration on the lysenin channel’s voltage regulated gating, using both multivalent and monovalent metals. A model was developed to explain the apparent subunit cooperativity within the lysenin channel. The model allows for the complex reaction to changing concentration of metal ions, and offers knowledge of the lysenin channel’s internal workings.
Clark, Tyler; Bryant, Sheenah; Rossland, Steve; Krueger, Eric; Hanna, Charles; and Fologea, Daniel, "Voltage Gating Interactions of the Protein Lysenin with Metal Ions in an Artificial Lipid Bilayer" (2014). College of Arts and Sciences Presentations. Paper 59.